Recognising animals based on distinctive body patterns, such as stripes, spots, or other markings, in night images is a complex task in computer vision. Existing methods for detecting animals in images often rely on colour information, which is not always available in night images, posing a challenge for pattern recognition in such conditions. Nevertheless, recognition at night-time is essential for most wildlife, biodiversity, and conservation applications. The SPOTS-10 dataset was created to address this challenge and to provide a resource for evaluating machine learning algorithms in situ. This dataset is an extensive collection of grayscale images showcasing diverse patterns found in ten animal species. Specifically, SPOTS-10 contains 50,000 32 x 32 grayscale images, divided into ten categories, with 5,000 images per category. The training set comprises 40,000 images, while the test set contains 10,000 images. The SPOTS-10 dataset is freely available on the project GitHub page: this https URL by cloning the repository.